Since 1999, we have been involved in preparing ectomycorrhizal grafts from living mycelium. For a few years, we have been involved in preparing such grafts from truffle mycelia. Those which we have found to be worth our effort are the species regarded as be the best and the most expensive by consumers and by the market.

Therefore, we prepare grafts from the following kinds of truffles:
– the white truffle (Tuber magnatum pico)
– the summer truffle (T. aestivum)
– the whitish truffle (T. borchii)
– the black truffle (T. melanosporum)
– the brumale truffle (T. brumale)
– the mesenteric truffle (T. mesentericum)

Since recently, our collection has included the mycelium of the desert truffle (Terfezia boudieri), obtained from an ascocarp from the United Arab Emirates.


Truffles are a perfect and very important ectomycorrhizal fungus. Therefore, they form ascocarps exclusively in symbiosis with the suitable trees and certain shrubs. They can form mycorrhiza with several species of deciduous and coniferous trees, however, they form ascocarps only with the certain ones, and only in appropriate habitat conditions. Pure truffle mycelia offered in grafts are isolated both from ascocarps, and the roots of trees from a natural truffle ground, thanks to which they are found in the company of helper fungi. Such a combination guarantees effective micorrhization, and, as the result of that, collecting ascocarps. As mycorrhizal fungi, they fulfill important functions of bioregulators in the environment as well. Grafts have the form of living hyphae in an aqueous-gel suspension. The mycelium frequently forms sporangia as soon as in a graft, which additionally increases its ability to inoculate. The grafts are offered for sale exclusively with the procedure of application because performing this procedure incompetently may fail to cause micorrhization. After the hitherto performed procedures of the application of our truffle grafts, the mycological analyses of the roots showed the presence of the mycelium of the previously-implanted truffles. That means that the grafts in use are effective, and introducing the mycelia of the demanded truffle is possible both on a plantation (hazel, nut, olive, etc.), and in a forest habitat, where truffles have disappeared.

Finally, a few important explanations concerning cultivation:

– Truffles require a very specific habitats; that should be a soil with quite a high pH, rich in microelements and humus. Requirements may differ depending upon the species of truffles.

– The best solution is to locate the plantations of plants which grow quite rapidly, and which are largely compatible  with truffles such as for example hazel or willow and poplar (too!), but in combination with biocenotic species such as Quercus sp., Carpinus sp., Acer sp. and others because truffles form micorrhizis with several species of trees.

– We recommend that plants be planted first, and, that a graft is applied when they have leaves. Then, we avoid costly procedures on dead plants, and mycelium is fed on assimilates. Of course, we do it on a specially-prepared field.

– An important element is appropriate solar irradiance, which is required by the mycelium if ascocarps are to be formed.

– So as to find ascocarps, it is necessary to use a trained dog, preferably one’s own.

Truffles in Poland; the possibilities of the cultivation of these noble fungi exist on the area of Poland as well, where there are rendzinas and similar soils. It is possible to graft truffles into the same trees which co-exist with them in nature, but mainly quickly-growing species such as: hazel, poplar, willow, and even princess tree. The species which may form ascocarps, in particular in the southern regions of Poland, are: Tuber aestivum, T. borchii, T. mesentericum. Of course, the one most recommended is the summer truffle (T. aestivum).

Details concerning orders and cultivation are available on the phone. Micorrhization is performed by us on order.

Our truffles in Croatia!

Two years ago we performed inoculation of hazelnut and walnut plantation with living mycelium of truffles (Tuber magnatum + T. aestivum) located in Slavonia region (Croatia) close to Novska village.

At the end of March 2014 we examined results of mycorrhization with my friend Zdravko Kvesič who is known mycologist and truffle expert. After relatively wet and warm winter we expected to find first truffle fruit bodies but sounders of wild boars came there earlier!!! Zdravko concluded with 100% certainty that truffles have already started to produce fruit bodies because of boars’ trails. Animals burrowed regular circles around young inoculated trees of 6 m diameter and depth around 40 cm. Therefore it seems absolutely necessary to fence plantation if you want to harvest truffles from your plantation.

Our observations have fully confirmed effectiveness of inocula produced by MYKOFLOR because only two years after inoculation fruit bodies of white truffles (Tuber magnatum “pico”) were developed. After Eastern we will start to return to this plantation with  trained dogs.

Robert Ślęzak



Truffles in Serbia!

We have got order for inoculation of hazelnut plantations in Republic of Serbia (outskirts of Sombor – Vojevodina region). In total 4 ha have been inoculated with mixed inoculum of Tuber aestivum and T.melanosporum.

We were in Serbia on 10th September 2013 and our team (Patrycja Szelągowska, Jurek Nieswadba, Andrzej Klasa and me) has been performed inoculation. Inoculation has been done in a good speed, hazelnuts plants were young (one or two years old). Plantation was established on good quality soil without stones however was infested by weeds. We will test effectiveness of inoculation in the next year and we are sure that first black truffles can be picked in 2016. Next mycorrhizations are in close future.

Włodzimierz Szałański